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KMID : 0545120110210060613
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 6 p.613 ~ p.616
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Biosynthesis of Glycosylated Derivatives of Tylosin in Streptomyces venezuelae
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Han Ah-Reum
Park Sung-Ryeol
Park Je-Won
Lee Eun-Yeol
Kim Dong-Myung
Kim Byung-Gee
Yoon Yeo-Joon
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Abstract
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Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
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KEYWORD
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tylosin, combinatorial biosynthesis, Streptomyces venezuelae
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